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Think Something’s Off With Your Hormones? These Are the Labs You Need to Ask For + My Journey with High Prolactin

If you’ve ever felt like something was off in your body—your period goes missing, your mood is all over the place, your skin is breaking out, or you’re exhausted for no reason—you are not crazy. Hormonal imbalances are real, and the only way to know what’s actually going on is to test.

Unfortunately, a lot of doctors will run a basic hormone panel (if that) or brush off symptoms as “stress” or “normal” without digging deeper. So if you suspect something’s off, here are the labs you need to advocate for. Screenshot this list, bring it to your provider, and ask for a full workup.

PS. If you are stopping birth control- A lot of doctors will want to wait 2-3 months if you stop birth control to allow your body to readjust. This is normal, but 3 months is the maximum time you should wait and allow for your hormones to balance out on their own. After that, it is best to get hormone testing done.

The Hormone Lab List:

Prolactin – High prolactin can signal a benign tumor called a prolactinoma, but can also be caused by stress, medications, or even thyroid dysfunction. Elevated levels can lead to missed periods, infertility, and low libido.

Estradiol (E2) – Measures your main form of estrogen. Low levels can mean hypothalamic amenorrhea; high levels can point to estrogen dominance or cysts.

Progesterone – Essential for cycle regulation, fertility, and mood balance. Test on day 19-21 of a 28-day cycle to assess ovulation.


LH & FSH – These pituitary hormones control your cycle. An elevated LH:FSH ratio may indicate PCOS.

Free & Total Testosterone – High levels may cause acne, hair thinning, and irregular periods. Important to assess for PCOS or androgen excess.

DHEA-S – An adrenal hormone that can also contribute to androgen excess and should be tested alongside testosterone.

TSH, Free T3, Free T4, and Thyroid Antibodies (TPO, TGAb) – Thyroid dysfunction is one of the most overlooked causes of hormonal imbalances, hair loss, fatigue, and irregular cycles.

Cortisol (AM blood draw or DUTCH test) – Measures your body’s stress hormone. Chronic high or low cortisol can affect every other hormone in your system.

Insulin & Fasting Glucose – Blood sugar imbalances drive hormonal chaos. These help identify early signs of insulin resistance or prediabetes.

SHBG (Sex Hormone Binding Globulin) – Helps interpret your testosterone and estrogen levels by showing how much is actually available for your body to use.

Now let’s talk more about my personal experience with high prolactin—what I’m doing lifestyle-wise, the supplements I take specifically to support it, and the medication I’m using (with a plan to taper off eventually).

First of all, finding out I had elevated prolactin levels was validating. I had been experiencing irregular cycles for over a year and I knew something wasn’t right. Surprisingly, I was seeing a holistic practitioner who kept wanting to test for other biomarkers or told me to wait it out. I had a gut feeling something else was wrong, so I made an appointment with my OBGYN and asked her if we could do hormone testing, and include Prolactin.

I did my labs, and my prolactin came back high. So we re-tested just to be sure, and it was still high. I had to get referred out to an endocrinologist because like I said in my video, one of the causes of high prolactin is a benign tumor. So it is important to get an MRI and rule that out.

After getting an MRI (to rule out a prolactinoma), I learned my elevated levels were likely due to long-term birth control use and chronic stress from my lifestyle. (Fasting excessively, high intensity workouts & running three miles every morning while fasting, not eating enough protein, and I went through a very emotionally draining time two summers ago which really shocked my system- among many other things!)

Right now, I’m taking a low dose of cabergoline, which is the gold-standard dopamine agonist used to lower prolactin levels. It’s been effective for me, but I’m working closely with my endocrinologist on a plan to taper off once my levels stabilize and my body is more regulated.

Alongside that, here’s what I’m doing naturally to support my body:

Inositol powder daily to help with hormone balance, blood sugar regulation, and overall mood support

Chill Pills (my favorite) to regulate cortisol and support my nervous system’s response to stress.

Magnesium at night to calm my body and support better sleep (which helps with hormone repair)

I will start Vitex berry once I am going to taper off my medication in the future. Vitex berry (chaste tree extract) helps support your cycle and naturally regulates prolactin through pituitary support.

Prioritizing food before caffeine, walking daily, and changing the way I work out—no more fasted, high-intensity workouts. I focus on movement that supports my hormones, especially during my luteal and menstrual phases

I’ve also made huge lifestyle changes—protecting my peace, setting boundaries, and rebuilding my nervous system from the ground up.

If your prolactin is high and you don’t have a tumor, this is the stuff no one tells you. Medication can help—but it’s the lifestyle changes that make the biggest difference. Be your own advocate, stay curious, and trust your intuition. You deserve answers and support.

If you’re going to invest in any form of hormone testing, make sure it’s comprehensive. A single test doesn’t give the full picture. Once you have results, you can work with a practitioner to actually treat the root cause—instead of guessing.

Trust yourself, ask questions, and advocate for your health. If something feels off, it probably is—and you deserve answers!

When to Take Your Supplements: A Pharmacist’s Guide to Timing for Maximum Results

There’s nothing more frustrating than investing in high-quality supplements only to take them at the wrong time and see minimal results. As a pharmacist with a background in functional nutrition, I’m here to tell you: timing matters.

Whether you’re taking magnesium to relax, inositol to balance hormones, or vitamin D to support immunity, the time of day and what you take your supplement with can make a huge difference. Here are the 30 most common supplements and exactly when to take them for optimal absorption and benefit!

Vitamin D3 + K2
Take with: fat-containing meal
Best time: morning or early afternoon
Why: Supports immune and hormone health; better absorbed with dietary fat and won’t interfere with melatonin at night.

Magnesium (glycinate, threonate, citrate, etc.)
Take: away from high-fat/fiber meals and caffeine
Best time: evening
Why: Supports sleep, stress, and regularity; works best at night when cortisol is naturally declining.

B-complex vitamins
Take: on empty stomach or 30 min before caffeine
Best time: morning
Why: Energizing and water-soluble—best taken alone to maximize absorption.

Omega-3s (EPA/DHA)
Take with: fat-containing meal
Best time: lunch or dinner
Why: Fat increases absorption; helpful for hormones, mood, and inflammation.

Inositol (myo-inositol or blend)
Take with: coffee, matcha, or water
Best time: morning or evening
Why: Balances insulin and hormones; tastes sweet, easy to pair with a beverage.

Creatine monohydrate
Take with: carbs + protein
Best time: post-lift or post-workout
Why: Insulin spikes improve creatine uptake; aids strength, brain health, and lean mass.

Collagen peptides
Take with: vitamin C or post-workout
Best time: mid-morning or post-exercise
Why: Collagen synthesis requires vitamin C; great for joints, skin, gut, and recovery.

Electrolytes (sodium, potassium, magnesium)
Take: on empty stomach
Best time: first thing in the morning
Why: Replenishes minerals, regulates energy and fluid balance.

CoQ10 (ubiquinol)
Take with: fat-containing meal
Best time: morning or afternoon
Why: Boosts mitochondrial energy; fat increases absorption.

Probiotics
Take: on empty stomach or 30 min before meals
Best time: AM or PM
Why: Helps microbiome and digestion; less competition with food improves survival.

Berberine
Take: 15–30 min before meals
Best time: before lunch and dinner
Why: Regulates blood sugar, insulin, and cravings; avoid pairing with probiotics.

Iron
Take with: vitamin C
Avoid with: calcium, caffeine, magnesium
Best time: late morning or evening
Why: Enhances absorption; best taken solo or with a light fruit like oranges.

Saffron extract
Take: before meals
Best time: lunch and dinner
Why: Stabilizes mood and reduces appetite; great for PMS or emotional eating.

Ashwagandha
Take with or without food
Best time: PM or before bed
Why: Helps cortisol regulation, stress, and deep sleep.

Zinc
Take with: food
Avoid with: calcium, iron
Best time: lunch or dinner
Why: Hormonal and immune benefits; can upset the stomach without food.

Chromium picolinate
Take with: any meal
Best time: breakfast or lunch
Why: Helps regulate blood sugar and reduce cravings.

NAC (N-acetyl cysteine)
Take: on an empty stomach
Best time: morning or mid-afternoon
Why: Powerful antioxidant that supports liver and detox pathways.

L-theanine
Take: with or without food
Best time: morning + afternoon
Why: Calms the nervous system, makes you feel more creative, and enhances focus without any stimulating effects.

Rhodiola Rosea
Take: on an empty stomach
Best time: morning
Why: Adaptogen for energy and mood; uplifts the body

L-theanine and Rhodiola are the main ingredients in our Chill Pills!

Vitamin C
Take: with or without food
Best time: morning or afternoon
Why: Supports immunity and collagen production; water-soluble.

DIM (Diindolylmethane)
Take: with food
Best time: lunch or dinner
Why: Helps with estrogen metabolism and hormonal balance.

Calcium
Take: with food
Avoid: iron supplements
Best time: evening
Why: Absorption is better with meals and pairs well with magnesium for bone health.

Melatonin
Take: on empty stomach
Best time: 30–60 min before bed
Why: Signals the body it’s time to sleep; not ideal long-term.

Glutathione (liposomal or NAC precursor)
Take: on empty stomach
Best time: morning
Why: Supports detox, oxidative stress, and cellular repair.

Taurine
Take: with or without food
Best time: pre-workout or PM
Why: Nervous system and bile support; calming or energizing depending on timing.

L-carnitine
Take: with carbs
Best time: pre-workout or AM
Why: Improves mitochondrial fat burning and endurance.

Resveratrol
Take with: fat
Best time: AM
Why: Antioxidant that supports longevity and metabolic health.

Turmeric/curcumin
Take: with black pepper and fat
Best time: with meals
Why: Reduces inflammation; needs fat and piperine to absorb well.

GABA
Take: on empty stomach
Best time: before bed
Why: Calming neurotransmitter that supports sleep onset and relaxation.

L-tyrosine
Take: on an empty stomach
Best time: morning
Why: Precursor to dopamine and thyroid hormone; great for focus and energy.

Timing your supplements right can completely change how effective they are. It’s not just about what you take—it’s about how and when you take it. Small changes like spacing your magnesium away from caffeine or pairing your iron with vitamin C can be the difference between “meh” and game-changing results.

Remember: consistency matters more than perfection, and you don’t need to overhaul your whole routine overnight. Start with a few key shifts, stay consistent, and track how you feel. Your body will thank you!